Figure 2. The overlay of elution profiles of the purified T6-D-suc, T6-L-suc and the mixture of synthesized T6 Lα, T6 Lβ, T6 Dα, T6 Dβ peptides.

Column: Shiseido UG 80 3.0×250 mm. Gradient: 17–26.5% acetonitrile/0.1% TFA over 25 min. Flow rate: 0.5 mL/min. Detection: 215 nm. a: elution profile of mixture of synthesized T6 Lα, T6 Lβ, T6 Dα and T6 Dβ. b: elution profile of purified T6-L-suc. c: elution profile of purified T6-D-suc.