Figure 4. The overlay of elution profiles of incubated T6-D-suc peptide after incubation for 0–80 min.

Column: Shiseido UG 80 3.0×250 mm. Gradient: 17–26.5% acetonitrile/0.1% TFA over 25 min. Flow rate: 0.5 mL/min. Detection: 215 nm. a: elution profile of T6-D-suc peptide at incubation time = 0 min. b: elution profile of T6-D-suc peptide which was incubated at 37°C for 20 min. c: elution profile of T6-D-suc peptide which was incubated at 37°C for 80 min.