Figure 5. Increase of α- and β- Asp with hydrolysis of succinimide.

Figure 5a: increase of L-α-Asp with hydrolysis of T6-L-suc peptide at 37°C. Figure 5b: increase of L-β-Asp with hydrolysis of T6-L-suc at 37°C. Figure 5c: increase of D-α-Asp with hydrolysis of T6-D-suc peptide at 37°C. Figure 5d: increase of D-β-Asp with hydrolysis of T6-D-suc peptide at 37°C. We calculated the rate constant for hydrolysis of succinimide at 37°C (k2, k4, k6 and k8) by using fitted curves in this figure.