Figure 7. Decrease of 4 Asp isomers at 70°C.

Figure 7a: decrease of L-α-Asp with incubation of T6-Lα peptide at 70°C. Figure 7b: decrease of L-β-Asp with incubation of T6-Lβ peptide at 70°C. Figure 7c: decrease of D-α-Asp with incubation of T6-Dα peptide at 70°C. Figure 7d: decrease of D-β-Asp with incubation of T6-Dβ peptide at 70°C. We calculated the rate constant for dehydration from isomers to succinimide at 70°C (k1, k3, k5 and k7) by using fitted curves in this figure. The rate constants at 50, 60, 80 and 90°C were also determined by the same method.