Figure 4. Supervised discrimination analysis integrated with the spectral index method identified a 3-protein signature that robustly segregated VAP⁺ from VAP^–.

Panel A: K-median clustering was performed based on the expression profiles of these 3 proteins, resulting in the correct classification of 27 out of 30 patients. Panel B: ROC analysis demonstrates the superior performance of the combined protein classifier compared to each individual member.