Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Mar 7;8(3):e58731. doi: 10.1371/journal.pone.0058731

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

PMC Copyright notice

a.) CSMD1 mRNA expression comparison in HCT116 WT and HCT116 DNMT1/3B DKO cDNA along with K562 and fetal brain cDNA. b.) The high-resolution melt curve analysis distinguishes between methylated and unmethylated CG at Reference position 3265577 based upon their melt temperature. Tumor 517 was mutated at CG3265577 so the melt peak coincided with HCT-DKO whereas methylated tumors coincided with the HCT-116 peaks, which is our positive control c.) The percentage of colorectal tumors methylated at CSMD1 loci determined by methylation specific PCR melt curve analysis.