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. 2013 Mar 7;8(3):e58696. doi: 10.1371/journal.pone.0058696

Figure 1. Effect of pUCOE vectors in the production of MB12/22 miniantibody.

Figure 1

CHO-S cells were transfected with pUCOE or pMB plasmid vector containing MB12/22 encoding sequence. Miniantibody expression levels and specificity were monitored in the culture medium collected 72 hours after transfection analyzing the binding of the recombinant antibody to C5 but also on C3 and BSA by ELISA (A). Miniantibody activity was also controlled analyzing inhibition of human and rat C5 activity in the activation of the classical pathway of the complement system (B). Values are the mean ± SD.