Figure 2. Detection of fibrillar Aβ_1–42 induced Aβ_1–40 production by flow cytometry.

For the analysis of Aβ_1–40 synthesis in amyloidic condition, Hep-1 cells were treated with fibrillar Aβ_1–42 in the presence (A) or absence (B) of serum, stained with anti Aβ_1–40 antibodies (biotinylated) and streptavidin conjugated phycoerythrin. Histograms are shown for the control with no added amyloid (ctrl) and for cells incubated with various concentrations of Aβ_1–42.as indicated in the figure.