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. 2013 Mar 7;8(3):e58698. doi: 10.1371/journal.pone.0058698

Figure 5. Lack of coexpression of leptin receptor (LepR) and Kiss1 before puberty.

Figure 5

A-F. Fluorescence photomicrographs showing the distribution of Kiss1 (hrGFP) and LepR (tdTomato) in prepubertal (21 days of age, P21) (A, C, E) and ovariectomized adult female mice (B, D, F). Note the lack of colocalization of Kiss1 and LepR in prepubertal mice and the higher colocalization rate in ovariectomized adult female mice (arrows indicate dual labeled neurons). Scale bar: A–F  =  200 µm. 3V, third ventricle; ME, median eminence.