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. 2013 Mar 7;8(3):e57393. doi: 10.1371/journal.pone.0057393

Figure 2. Effects of Wnt11 and Wnt5a in tumour development.

Figure 2

A: Effects of Wnt11 overexpression and suppression in A549 AC cell line. Gene expression of A549-GFP and A549 treated with control siRNA served as reference, respectively. Note the decreased expression of E-cadherin in Wnt11-A549 (p<0.011), and the increased E-cadherin level following siWnt11 treatment (p<0.006). B: Recombinant Wnt11 treatment of SAEC cultures. Gene expression of untreated control cells was used as reference. Note the concentration dependent “cadherin switch” upon rWnt11 treatment. (Data are representative of five independent experiments where SAEC was used of five individual donors of different ages and sexes). C: Expression of E- and N-cadherin in primary adenocarcinoma lung tissue sample compared to its healthy autologous control pair. Data are representative of the analysis of three independent tissue pairs. D: Recombinant Wnt5a treatment of SAEC cultures. Gene expression of untreated control cells was used as reference. Note the concentration dependent “cadherin switch” upon rWnt5a treatment. (The presented data are representative of five independent experiments where SAEC was used as control of five individual donors of different ages and sexes). E: Invasion assay. The A549 AC cell line that expresses high levels of Wnt11 is more invasive than the H157 SCC cell line with lower expression of Wnt11 (p<0.008). F: Invasion assay. Primary non-cancerous SAEC were treated with 1 µg/ml rWnt11 and rWnt5a for three days prior to the assay. Only the rWnt5a treated SAEC migrated faster than the non-treated control cells. (The results are representative of three independent experiments where SAEC was used from three individual donors of different ages).