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. 2013 Mar 7;8(3):e57393. doi: 10.1371/journal.pone.0057393

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© 2013 Bartis et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Immunofluorescent staining of SAEC. B: Immunofluorescent staining of normal A549. C: Immunofluorescent staining of Wnt11 overexpressing A549. D: Immunofluorescent staining of H157 monolayer cell cultures. (60x image, red: β-catenin, blue: DAPI). Note the dramatic increase in nuclear localization and the decrease in cellular membrane localization of A549 AC, Wnt11-A549 and H157 SCC cell lines compared to the normal pulmonary epithelium (SAEC). Data presented are representative of three independent experiments. E: Densitometry of immunofluorescent images of SAEC, A549, Wnt-11-A549 and H157 cells. Note the increased nuclear localization of β-catenin particularly in the Wnt11-A549 cell line. (M: cellular membrane, CS: cytosol, N: nucleus).