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. 2013 Mar 7;8(3):e58034. doi: 10.1371/journal.pone.0058034

Figure 5. Comparative expression of uridine phosphorylase in wild-type and pyrimidine auxotrophic trypanosomes.

Figure 5

Expression of uridine phosphorylase (UP) was assessed by Real Time PCR in WT and PYR6-5 −/− strains grown under various conditions. The results are presented normalized to the control (group1) and are the average and SE of 8 replicates. 1. Control: WT grown in HMI-9; 2. WT grown 48 h in HMI-9-tmd+100 µM uracil; 3. WT grown 48 h in HMI-9-tmd+1 mM uridine; 4. PYR6-5−/− grown 48 h in HMI-9-tmd+100 µM uracil; 5. PYR6-5−/− grown 48 h in HMI-9-tmd+1 mM uridine; 6. PYR6-5−/−long-term adapted to growth on uridine, grown 48 h in HMI-9-tmd+100 µM uracil; 7. PYR6-5−/−long-term adapted to growth on uridine, grown 48 h in HMI-9-tmd+1 mM uridine. Data were analysed with a one-way ANOVA with Tukey's correction. Horizontal asterisks indicate significant differences from control; vertical asterisks indicate significant differences between individual bars as indicated.