Table 1. EC50 values for some pyrimidine analogues tested on WT s427 and PYR6-5 −/− bloodstream forms grown in standard HMI-9, using a standard protocol based on the fluorescent indicator dye Alamar Blue.
| WTs427 | PYR6-5 −/− | RF | P value | |||
| AVG ± SE(µM) | n | AVG ± SE(µM) | n | |||
| 5-Fluorouracil | 35.9±1.5 | 4 | 2.3±0.07 | 4 | 0.06 | <0.001 |
| 5-Fluoro-2′deoxyuridine | 4.6±0.5 | 3 | 0.77±0.10 | 3 | 0.16 | 0.002 |
| 5-Fluoro-2′deoxycytidine | 43.7±4.4 | 3 | 4.6±0.9 | 3 | 0.105 | <0.001 |
| 5-Fluorouridine | >5000 | 5 | 472±3 | 3 | <0.09 | <0.001 |
| 5-Fluoroorotic acid | 13.2±1.2 | 3 | >5000 | 4 | >380 | <0.001 |
RF, resistance factor, being the ratio of the EC50 values (µM) for knockout over WT strains. P value is based on an unpaired Students t-test.