Table 1. Melting temperatures of URN1-FF at different pHs.
Tm (K) | ||
CD1 | Intrinsic fluorescence2 | |
pH 2.0 | 313.5±1.3 | 310.9±0.3 |
pH 2.5 | 321.5±0.1 | 318.9±0.1 |
pH 3.0 | 332.8±0.1 | 335.8±0.1 |
pH 5.7 | 340.9±0.3 | 341.4±0.1 |
Changes in molar ellipticity were monitored at 222 nm. 2 Changes in intrinsic fluorescence were monitored at 350 nm.