Table 1. Statistics of the nine microsatellite loci.
Locus | MgCl2 (mM) | AT1/AT2(°C) | Na | Size | HO | HE |
GATA98a | 4.7 | 51°/52° | 9 | 74–120 | 0.69 | 0.73 |
GT541b | 3.1 | 56°/55° | 9 | 79–99 | 0.7 | 0.76 |
AC137b | 3.1 | 55°/54° | 9 | 91–119 | 0.64 | 0.66 |
GT023c | 3.1 | 60°/58° | 6 | 114–124 | 0.77 | 0.76 |
CA232b | 3.1 | 56°/55° | 8 | 142–168 | 0.65 | 0.65 |
AC087b | 3.1 | 56°/55° | 12 | 165–180 | 0.83 | 0.83 |
EV037d | 3.9 | 51°/52° | 8 | 172–194 | 0.58 | 0.58 |
GATA417a | 3.1 | 56°/55° | 13 | 174–226 | 0.83 | 0.83 |
CA234b | 3.1 | 55°/54° | 13 | 191–215 | 0.91 | 0.88 |
Mean | 9.6 | 0.74 | 0.74 | |||
SD | 2.4 | 0.03 | 0.09 |
At = Annealing temperature (subscript 1 and 2 correspond to the first and second PCR cycle). Na = Number of alleles per locus, Size = observed range in fragment size in base pairs (bp), Observed (HO) and Expected (HE) heterozygosity per locus. HO deficiency loci are highlight in bold (p<0.05).
36 Palsbøll et al., 1999,
34 Bérubé et al. 2005,
37 Bérubé et al. 200,
38 Valsecchi and Amos 1996.