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. 2013 Mar 7;8(3):e57865. doi: 10.1371/journal.pone.0057865

Figure 3. The interactions of the three srGAPs.

Figure 3

A–D. Non-tagged srGAP3 and GFP-tagged wild/mutated srGAPs or deletions were co-transfected into HEK293FT, and immunoprecipitated with GFP antibody. (A) Lane1: Mock; lane2: srGAP3-WT. (B) Lane1: Mock; lane2: srGAP1-WT. (C) Lane1: Mock; lane2: srGAP2-WT; lane3: srGAP2R527A. (D) Lane1: srGAP2-IF-BAR; lane2: srGAP2-ΔIF-BAR. E. Non-tagged srGAP3 and GFP-tagged wild type srGAP2 were co-transfected into HEK293FT, and then immunostained with srGAP3 antibody (3A1). The pearson's coefficient of Correlation (short for r) is a measure of the degree of colocalization of srGAP2 and srGAP3. The left panels, upper: one type cell with no filopodia; lower: the other type cell with numerous filopodia. Arrowhead: srGAP2 (+) and srGAP3 (−); arrow: srGAP2 (+) and srGAP3 (+). Bar = 5 µm. F. Neuro2a lysates were immunoprecipitated with srGAP2 (2A2) and srGAP3 (3A1) antibodies, and then blotted with another srGAP3 antibody, 3A3.