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. 2013 Mar 7;8(3):e57865. doi: 10.1371/journal.pone.0057865

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© 2013 Ma et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

A. RhoGAP pull-down assay by GST-CA Cdc42, Rac1 and RhoA was used to precipitate three GFP-tagged srGAPs (WT) and their RhoGAP mutants (RA) in transfected HEK293T cells. P190A RhoGAP was selected as a positive control. B. RhoGAP pull-down assay was performed to precipitate endogenous srGAP2 in Neuro2a cells. The amount of GST-fusion protein used in the assay was revealed by naphthol blue black staining (lower). C. RhoGAP pull-down assay was performed to precipitate endogenous srGAP1-3 from P15 rat cerebral cortex.