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. 2013 Mar 7;8(3):e57865. doi: 10.1371/journal.pone.0057865

Figure 7. Slit-Robo signaling involves in neuronal differentiation of Neuro2a cells.

Figure 7

A. Robo1-Fc or Robo1ecto-TM plasmids were transfected into Neuro2a cells, and cell differentiation rate was analyzed. n = 3, Mean ± S.D., one-way ANOVA, *P<0.05. B. A working model proposed that srGAPs proteins may regulate NB cell differentiation as a mediator of Slit2-Robo1 signaling pathway. The IF-BAR domain might localize srGAPs to the plasma membrane, where they interact with Robo1 through their SH3 domain. Slit2 binds to Robo1 receptor and subsequently activates srGAP3 RhoGAP activity. Downregulation of GTP-bound Rac1 activity leads to inhibition of neuronal differentiation in Neuro2a cells.