Table 2.
Roles of NER and TLS in formaldehyde mutagenesis
| Forward Mutation (CAN1) Median Mutant Frequency, 10−6 |
Frameshift Reversion (lys2ΔA746) Median Mutant Frequency, 10−8 |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| NER, TLS | control | HCHO- | -fold | control | HCHO- | -fold | |||||
| Genotype | na | (sham) | (95% CI) | treated | (95% CI) | changeb | (sham) | (95% CI) | treated | (95% CI) | changeb |
| wild-type | 24 | 1.73 | (1.09 –2.18) | 1.69 | (1.01 – 2.09) | 1.0 | 0.43 | (0 – 0.63) | 0.32 | (0 – 0.80) | 0.7 |
| rad14 | 23 | 2.95 | (1.85 – 4.56) | 6.24 | (5.06 – 8.40) | 2.1 | 0.60 | (0 – 2.31) | 2.00 | (0.89 – 3.31) | 3.3 |
| rad14 rev3 | 16 | 0.48 | (0.26 – 1.07) | 0.57 | (0.33 – 1.32) | 1.2 | 0.43 | (0 – 1.68) | 0.82 | (0 – 1.37) | 1.9 |
| rad14 rad30 | 24 | 2.61 | (1.90 – 3.29) | 5.58 | (4.43 – 6.76) | 2.1 | 0.78 | (0 – 1.50) | 1.59 | (0.77 – 4.36) | 2.1 |
| rad14 rad30 rev3 | 24 | 0.72 | (0.56 – 1.27) | 0.74 | (0.45 – 1.12) | 1.0 | 0.49 | (0 – 0.96) | 0.52 | (0 – 1.60) | 1.0 |
a
Number of independent cultures split into two and subjected to sham vs. formaldehyde treatment
b
Ratio of treated:control mutant frequencies