FIGURE 1.

SLN alone is responsible for muscle thermogenesis. A, maintenance of T_c in WT, PLB^−/−, and SLN^−/− mice with or without iBAT exposed to 4 °C for a period of 8 h. Data are mean ± S.E. of 4–10 animals; ***, p < 0.001 versus WT; ns, not significant as analyzed by Student's t test. B, Ca²⁺ dependence of Ca²⁺ uptake was measured in samples expressing SERCA1 alone or with SLN. Untransfected HEK cells were used as control. The values are given as mean of the percentage of SERCA1 mean ± S.E. (n = 3–5 samples). C, the effect of SLN on ATP hydrolysis in the absence and presence of ionophore A23187. The values are given as mean of the percentage of SERCA1 mean ± S.E. (n = 3 samples).