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A and B, acute effect of RSV (1 h treatment) versus vehicle on P-p38 and P-AMPK protein expression. C and D, longer term (total 24 h) effect of RSV or NAM/RSV versus vehicle on AMPK and p38 (phospho/total) phosphorylation in quiescent or contracting (3 h followed by 21 h of recovery) myotubes (n = 9, arbitrary scanner units (A.U.) corrected for loading using GAPDH; *, p < 0.05, versus vehicle; ‡, p < 0.05, overall effect of treatment with no stimulation (Stim) versus control (Con)).
