TABLE 1.

ITC data

ND, no binding detected.

Cell	Injectant	n	KD	ΔH
			μm	kcal/mol
Lys48-diubiquitin	FAF1 UBA	0.79 ± 0.01	3.5 ± 0.2	−7.7 ± 0.64
UBL1-UBL2	FAF1 UBA		ND
UBX	FAF1 UBA		ND
VCP N	FAF1 UBX	1.13 ± 0.03	25.6 ± 2.1	−4.3 ± 0.20
VCP N	FAF1 (TFPR → AG)		ND
VCP N	Npl4 UBD	1.04 ± 0.02	17.8 ± 1.4	−5.1 ± 0.20
VCP ND1	FAF1 UBX	0.72 ± 0.05	22.7 ± 1.1	−5.0 ± 0.51
VCP ND1 + ATP	FAF1 UBX	0.61 ± 0.02	20.1 ± 1.7	−5.1 ± 0.33
VCP ND1D2	FAF1 UBX		ND
VCP ND1D2	FAF1 UAS-UBX		ND
VCP ND1D2 + Ufd1-Npl4	FAF1 UBX	0.23 ± 0.11	08.4 ± 0.1	−23.4 ± 13.83
VCP ND1D2 + Ufd1-Npl4 + ATP	FAF1 UBX	0.27 ± 0.08	09.6 ± 0.2	−18.3 ± 07.03
VCP ND1D2 + Ufd1-Npl4 + ATP	FAF1 UAS-UBX	0.25 ± 0.09	19.5 ± 4.2	−28.0 ± 16.47