FIGURE 4.

Western blot analyses of proteins critical for photosynthetic function, including polypeptides of PSI, PSII, the light-harvesting complex (LHCBM1), and ATP synthase polypeptides (AtpB). Chlamydomonas proteins from the WT, cpld38, and the various cpld38-CPLD38 rescued strains were resolved by SDS-PAGE on a 15% polyacrylamide gel, and various proteins were detected immunologically. Samples were taken for analysis when the strains were in their exponential growth phase in low light (40 μmol photons m⁻² s⁻¹) on TAP media with moderate shaking (150 rpm). The antibodies used for this analysis are to the polypeptides indicated on the right side of the figure. Samples were normalized by cell number (∼2 × 10⁵ cells per lane). α-Tubulin was used as a loading control.