TABLE 1.
In vitro and in vivo analysis of chlorophyll/cell ratios, chlorophyll/pigment ratios, maximum photosynthetic efficiency, and cytochrome b6f activity
Samples were taken for measurement during exponential growth in low light (40 μmol photons m−2 s−1) on TAP medium with moderate shaking (150 rpm).
| Chl/106 cellsa | PSI/PSII ratio | P700/Chlb | PSII/cellc | PSI/celld | Cyt f/Chle | FV/FM | PSI act/Chlf | Cyt b6f act/Chlg | |
|---|---|---|---|---|---|---|---|---|---|
| WT | 2.98 ± 0.41 | 0.8 | 0.88 ± 0.08 | 2.09 | 2.62 | 0.36 ± 0.06 | 0.66 ± 0.01 | 636 ± 44 | 156 ± 14 |
| cpld38 | 1.28 ± 0.27 | 0.93 | 1.12 ± 0.07 | 1.33 | 1.43 | 0.16 ± 0.06 | 0.68 ± 0.01 | 545 ± 61 | 17 ± 18 |
| cpld38-CPLD38_3 | 3.11 ± 0.3 | 1.06 | 0.96 ± 0.05 | 3.16 | 2.98 | 0.51 ± 0.08 | 0.66 ± 0.01 | 722 ± 27 | 94 ± 17 |
a Data were expressed in micrograms of total chlorophyll/106 cell; values are the average of 8–11 replicates ± S.D.
b Data were expressed in nanomoles/mg total chlorophyll; values are the average of 10 replicates ± S.D.
c The estimated value was determined from the chlorophyll/106 cells, PSI/PSII ratio, and P700/ chlorophyll (expressed in nanomoles of PSII/109 cell).
d The estimated value was determined from the chlorophyll/106 cells and P700/chlorophyll (expressed in nanomoles of PSI/109 cell).
e Data were expressed in nanomoles/mg of total chlorophyll; values are the average of 10 replicates ± S.D.
f Data were expressed in micromoles of O2 consumed per mg of chlorophyll/h; values are the average of three replicates ± S.D.
g Data were expressed in micromoles of O2 consumed per mg of chlorophyll/h; values are the average of 5–9 replicates ± S.D.