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. 2013 Jan 23;288(10):7077–7085. doi: 10.1074/jbc.M112.401190

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Immunocytochemical localization of FXYD2 in rodent pancreas. Representative confocal images of rat pancreatic islet double-labeled for insulin (A) and FXYD2b (B) are shown. Secondary antibodies were FITC-conjugated anti-guinea pig (A) and Alexa-555 anti-rabbit (B) antibodies. Co-localization is seen in the merged image (C). Counterstaining for nuclei (blue, C) was with To-Pro. Bar, 20 μm. D–F, shown is immunostaining of mouse pancreatic islet with polyclonal rabbit antibodies against α1 subunit of Na,K-ATPase (green, D) and FXYD2b (green, E). Secondary antibodies were donkey-anti-rabbit Alexa 488. F, shown is the negative control for secondary antibodies alone. Nuclei (D–F) were counterstained with To-Pro. Bars, 20 μm.