FIGURE 1.

Wild-type and mutant MeCP2 proteins and reporter constructs. A, purification of wild-type and mutant MeCP2 proteins. Shown is the polyacrylamide-SDS gel electrophoresis of full-length MeCP2 isoform 1 (also known as MeCP2A, MeCP2e2, and MeCP2β) as well as the indicated C-terminally truncated mutant proteins. The locations of the MBD and HDAC-dependent TRD are shown. B, reporter constructs. Two versions of the super core promoter (with sequences from −50 to +50 relative to the +1 transcription start site) were designed to contain either eight CG dinucleotides or 0 CG dinucleotides. These core promoters were inserted into pUC119 (which has 209 CG dinucleotides) or pCpG-Txn (which has 0 CG dinucleotides) to give the reporter constructs. In addition, the pCpG-SCP-Distal13CG construct has a stretch of 13 CG dinucleotides ∼1.7 kbp upstream of the SCP lacking CG dinucleotides.