FIGURE 4.

UNC93B1 mutant mice are more susceptible to infection with L. major than TLR3/7, TLR7/9, and TLR3/9 double KO mice. A, animals were infected subcutaneously in the right hind footpad with 1 × 10⁶ promastigotes. The course of infection was monitored weekly, and the diameter of the primary footpad lesions was measured. The mean size of lesions and S.E. are shown (five mice per group). Bar graphs correspond to the parasite load quantification in lesions by limiting dilution analysis at week 10 post-infection. Data shown are mean ± S.E. and are representative of three independent experiments. B, spleens were harvested from infected mice, and cells were cultured with no stimulation, under the stimuli of 1 × 10⁶ FTAg or Concanavalin A. Accumulation of IFN-γ and IL-10 in culture supernatants, 48 h after antigen stimulation, was evaluated by ELISA assay as described under “Experimental Procedures.” Results are expressed as mean and S.E. of duplicate measurements of four animals per group. The presented data are representative of three independent experiments.