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. 2013 Jan 28;288(10):7263–7270. doi: 10.1074/jbc.M112.418004

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Prolonged oxidation of PRL by TRP32 knockdown. U2OS cells were transfected with siRNA against TRP32 (siTRP32) and then treated with 200 μm H₂O₂ for the indicated time intervals. Cell lysates were subjected to nonreducing or conventional (reducing) SDS-PAGE followed by immunoblot analysis with the indicated antibodies (upper panels). The amounts of reduced PRL3 were quantified and indicated as a percentage of those of total PRL3 (lower panel). Arrowhead, reduced proteins; asterisk, oxidized proteins containing intramolecular disulfide bonds. siControl, siRNA negative control.