TABLE 1.
Constructs used in this study. Names of the plasmids as well as mutated region/residues are described
| Name | Construct | Mutated region or residue |
|---|---|---|
| Mock/vehicle | pCDNA3 | |
| WT | pCDNA3-ITK-SYK | |
| KD | pCDNA3-ITK-SYK-K262R | Invariant catalytic lysine |
| Y183F | pCDNA3-ITK-SYK-Y183F | Interdomain-B |
| Y208F | pCDNA3-ITK-SYK-Y208F | Interdomain-B |
| Y212F | pCDNA3-ITK-SYK-Y212F | Interdomain-B |
| Y208E | pCDNA3-ITK-SYK-Y208E | Interdomain-B |
| Y212E | pCDNA3-ITK-SYK-Y212E | Interdomain-B |
| Y385F | pCDNA3-ITK-SYK-Y385F | Activation loop |
| Y386F | pCDNA3-ITK-SYK-Y386F | Activation loop |
| Y385F/Y386F | pCDNA3-ITK-SYK-Y385F/Y386F | Activation loop |
| Y385E | pCDNA3-ITK-SYK-Y385E | Activation loop |
| Y386E | pCDNA3-ITK-SYK-Y386E | Activation loop |
| Y385E/Y385E | pCDNA3-ITK-SYK-Y385E/Y385E | Activation loop |
| Y489F | pCDNA3-ITK-SYK-Y489F | C terminus |
| Y490F | pCDNA3-ITK-SYK-Y490F | C terminus |
| Y491F | pCDNA3-ITK-SYK-Y491F | C terminus |
| Y489F/Y490F/Y491F | pCDNA3-ITK-SYK-Y489F/Y490F/Y491F | C terminus |
| Y489E/Y490E/Y491E | pCDNA3-ITK-SYK-Y489E/Y490E/Y491E | C terminus |
| SYK | pCDNA3-SYK | |
| SYK-KD | pCDNA3-SYK-K402R | Invariant catalytic lysine |
| Y525E | pCDNA3-SYK-Y525E | Activation loop |
| Y526E | pCDNA3-SYK-Y526E | Activation loop |
| ZAP-70 | pCDNA3-ZAP-70 | |
| ZAP-70-KD | pCDNA3-ZAP-70-K369A | Invariant catalytic lysine |
| Y492E | pCDNA3-ZAP-70-Y492E | Activation loop |
| Y493E | pCDNA3-ZAP-70-Y493E | Activation loop |
| SLP-76-myc | pMSCV-SLP-76-myc | C-terminal Myc tag |
| GFP-SLP-76 | pEGFP-SLP-76 | N-terminal GFP tag |
| GFP-SLP-76-Y3F | pEGFP-SLP-76-Y3F | N-terminal tag and Y112F, Y128F, and Y145F |