FIGURE 2.
Temporal variability in intracellular nickel sequestration in the roots of Alyssum species. Confocal micrographs showing intracellular sequestration of nickel in the roots of A. murale (A) and A. montanum (B) at treatments of 20 min (center panels) and 1 h (bottom panels). Controls (top panels) are representative images for no nickel treatments. The roots were treated with 700 μm Ni(NO3)2 and intracellular nickel was visualized with nickel specific dye Newport Green DCF (488 nm) as shown in left panels. Right panels show differential interference contrast (DIC) phase images. Scale bar = 5 μm.