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. 2013 Jan 15;288(10):7351–7362. doi: 10.1074/jbc.M112.406645

FIGURE 3.

FIGURE 3.

Transient influx of nickel ions in mitochondria and sequestration to vacuoles. Confocal micrographs showing divergence of fluorescence from nickel specific Newport Green DCF and mitochondria-specific MitoTracker Red in the roots of A. montanum (top panel) and A. murale (bottom panel). Dual emission confocal images of Newport Green DCF and MitoTracker Red CMXRos are shown in the left two panels. These images are overlaid in the two rightmost panels to show the deviation in the patterns of the labeled organelles (Overlay). Arrow bars in right panel indicate dual staining of mitochondria in the roots of A. murale. The roots were treated with 700 μm Ni(NO3)2 for 1 h before subjecting to staining with dyes. Scale bar = 10 μm.