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. 2013 Jan 15;288(10):7351–7362. doi: 10.1074/jbc.M112.406645

FIGURE 4.

FIGURE 4.

Confirmation and quantification of mitochondrial nickel influx in purified mitochondria. Confocal micrographs showing co-localization of fluorescence from nickel-specific Newport Green DCF and mitochondria-specific MitoTracker Red CMXRos in purified mitochondrial fractions from the roots of A. murale (A) and A. montanum (B). Dual emission confocal images of Newport Green DCF and MitoTracker Red CMXRos are shown in left two panels. For A. murale, the inset images show co-labeled enlarged mitochondria. These images are overlaid in the right panel to show the coincidence of the labeled organelles (Overlay). Scale bar, 10 μm. C, nickel content in root mitochondria of Alyssum species treated with 700 μm Ni(NO3)2 was estimated using ICP-AES. Quantitative data shows increased nickel content in mitochondria of A. murale compared with A. montanum plants. Data are expressed as nanamole of nickel mg−1 mitochondrial protein and represent the mean ± S.D. Data are representative of three experiments, p < 0.05, n = 6.