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. 2013 Jan 15;288(10):7351–7362. doi: 10.1074/jbc.M112.406645

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Comparison of the effects of nickel ions on Krebs cycle in the root mitochondria of Alyssum species. A, a kinetic investigation of citrate synthase (CS, EC 1.11.1.6) in the root mitochondrial fractions of Ni(NO₃)₂-treated (700 μm) hyperaccumulator A. murale and comparison with A. montanum. B, a kinetic investigation of malate dehydrogenase activity in the direction of NADH oxidation (MDH, EC 1.1.1.37) in the root mitochondrial fractions of Ni(NO₃)₂-treated (700 μm) hyperaccumulator A. murale and comparison with A. montanum. C, a kinetic investigation of malate dehydrogenase activity in the direction of malate oxidation (EC 1.1.1.37) in the root mitochondrial fractions of Ni(NO₃)₂-treated (700 μm) hyperaccumulator A. murale and comparison with A. montanum. Total enzymatic activities are expressed as nanamolar/min/mg of mitochondrial protein. Results are the average of three independent determinations ± S.D. *, indicates significant difference from control, p < 0.05).