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Workflow for the analysis of peripherome complexes. For the isolation of PIM complexes, the IMVs were treated independently, either with 2 m KCl or 0.34 mm DDM (2 times CMC) or sequentially, with KCl first and then DDM. Extracted native complexes were fractionated using size-exclusion chromatography. Fractions were pooled in six groups (as indicated) and further separated via N-PAGE. Bands of interest were excised, trypsinized, and analyzed via nanoLC-MS/MS.
