Fig. 4.

Analysis of PRR11 cell cycle regulation. A, An overview of the entire human PRR11 protein sequence including features and motifs is shown (top), with the known ubiquitination site and phosphorylation sites indicated. An alignment of the C-terminal region of the PRR11 protein is shown (bottom), with various motifs and relevant known post-translational modifications to the human protein indicated. The conservation of each sequence motif is indicated by a colored overlay. Red vertical bars in the protein sequence represent missing sequence or gaps removed to show homology. The human PRR11 protein is highlighted in the alignment. B, Fluorescence-activated cell sorting of asynchronous U2OS cells stained for DNA content and sorted into G1, S or G2/M-phases of the cell cycle based on the gates indicated, with corresponding percentages of the total population. Immunoblotting of total cell lysates from the starting asynchronous cells and the sorted G1, S and G2/M cells is shown, (n = 2). Arrow head (filled white) indicates the PRR11 band, and an asterisk marks a nonspecific band.