Table I. Results of the ANOVAs: sums of squares for abundance of enzymes and their isoforms involved in glycolysis, ethanol, acetate, and glycerol pathways. For some enzymes/isoforms. data transformation was necessary to obtain normally-distributed residues: log transformation for the fermentation proteome, Pgi1p-1660, Pfk2p-4832, Fba1p-4759, Tdh1p-2775, Tdh2p-4872, Tdh2p-4740, Tdh3p-all isoforms, Tdh3p-acidic, Tdh3p-intermediary, Tdh3p-basic, Pgk1p-basic, Gpm1p-all isoforms, Gpm1p-3333, Gpm1p-3345, Gpm1p-3313, Eno1p-all isoforms, Eno2p-all isoforms, Eno2p-intermediary, Eno2p-acidic, Pyk1p-1587, Pdc1p-1606, Adh1p-all isoforms, Adh1p-4799, Ald6p-1565; inverse transformation for Fba1p-all isoforms, Fba1p-4758, Eno1p-acidic, Pyk1p-all isoforms, Pyk1p-1630, Pyk1p-1310, Pdc1p-4854, Adh1p-4808; and finally square root transformation for Tdh1p-2824 and Eno1p-intermediary. Variance was calculated across the 27 strain x medium combinations.
| Metabolic pathway | Enzymes | Isoforms reference | % of total sum of squares (df)a |
Variance | |||
|---|---|---|---|---|---|---|---|
| medium (2) | strain (8) | medium × strain (16) | residual (52) | ||||
| Fermentation proteome | all AF enzymes | all isoforms | 3.55 | 10.84 | 28.86 | 56.74 | 6.21E-02 |
| Glycolysis | Pgi1p | 1660 | 0.33 | 19.9 | 17.9 | 61.87 | 1.86E-05 |
| Pfk1p | all isoforms | 2.2 | 34.9*** | 27.62 | 35.29 | 1.37E-05 | |
| intermediary | 2.57 | 43.99*** | 24.85 | 28.59 | 3.85E-06 | ||
| basic | 1.96 | 31.2** | 19.09 | 47.75 | 1.81E-07 | ||
| acidic | 5.12 | 27.45** | 28.87 | 38.57 | 2.02E-06 | ||
| Pfk2p | 4832 | 0.82 | 35.26** | 27.79 | 36.13 | 1.94E-06 | |
| Fba1p | all isoforms | 2.18 | 9.57 | 24 | 64.25 | 5.69E-04 | |
| 4759 | 2.14 | 21.07 | 26.3 | 50.5 | 5.23E-05 | ||
| 4758 | 0 | 8.3 | 31.08 | 60.62 | 4.87E-04 | ||
| 2414 | 9.22 | 17.38 | 16.95 | 56.45 | 9.35E-05 | ||
| Tpi1p | 3406 | 1.71 | 11.57 | 19.81 | 66.9 | 9.08E-05 | |
| Tdh1p | all isoforms | 18.73** | 21.9** | 20.82 | 38.55 | 3.31E-03 | |
| 2775 | 13.79** | 19.81* | 27.79 | 38.61 | 2.58E-04 | ||
| 2757 | 14.46* | 20.09* | 19.27 | 46.18 | 9.84E-04 | ||
| 2729 | 12* | 21.56** | 28.25 | 38.19 | 5.41E-05 | ||
| 2824 | 10.55 | 29.07** | 10.8 | 49.58 | 1.02E-04 | ||
| Tdh2p | all isoforms | 1.77 | 37.89*** | 24.82 | 35.52 | 1.71E-03 | |
| 4732 | 2.56 | 50.81*** | 11.24 | 35.4 | 7.68E-05 | ||
| 4872 | 4.21* | 77.63*** | 5.2 | 12.96 | 9.01E-05 | ||
| 4740 | 0.49 | 56.38*** | 17.4 | 25.73 | 1.73E-03 | ||
| Tdh3p | all isoforms | 1.3 | 14.91 | 33.52 | 50.27 | 1.63E-03 | |
| basic | 1.51 | 17.71 | 22.79 | 57.99 | 5.16E-05 | ||
| intermediary | 1.22 | 12.24 | 34.52 | 52.02 | 1.13E-03 | ||
| acidic | 1.28 | 79.49*** | 5.79 | 13.44 | 3.11E-04 | ||
| Pgk1p | all isoforms | 8.49* | 48.63*** | 9.48 | 33.39 | 2.68E-03 | |
| basic | 20.61*** | 34.57*** | 13.09 | 31.74 | 2.70E-05 | ||
| intermediary | 5.58 | 42.82*** | 11.97 | 39.63 | 1.61E-03 | ||
| acidic | 7.67* | 53.39*** | 5.53 | 33.41 | 9.13E-05 | ||
| Gpm1p | all isoforms | 9.22 | 6.03 | 23.08 | 61.67 | 2.98E-04 | |
| 3333 | 9.71 | 2.83 | 23.73 | 63.72 | 1.98E-04 | ||
| 3345 | 5.47 | 6.35 | 17.42 | 70.75 | 1.39E-05 | ||
| 3313 | 2.89 | 24.26** | 32.97 | 39.88 | 3.74E-05 | ||
| Eno1p | all isoforms | 27.55*** | 26.87*** | 18.24 | 27.34 | 2.31E-03 | |
| basic | 27.34*** | 27.65*** | 15.64 | 29.37 | 6.54E-05 | ||
| intermediary | 21.82*** | 26.52** | 15.69 | 35.96 | 1.30E-03 | ||
| acidic | 16.07** | 31.99*** | 15.23 | 36.71 | 1.02E-04 | ||
| Eno2p | all isoforms | 3.39 | 27.88** | 20.81 | 47.92 | 1.88E-03 | |
| basic | 4.18 | 32.77*** | 21.16 | 41.9 | 5.08E-04 | ||
| intermediary | 1.52 | 22.85* | 32.17 | 43.46 | 1.38E-03 | ||
| acidic | 1.9 | 15.67 | 14.51 | 67.92 | 6.40E-05 | ||
| Pyk1p | all isoforms | 1.7 | 41.85*** | 19.56 | 36.89 | 2.99E-03 | |
| 1587 | 0.79 | 21.51 | 22.41 | 55.29 | 8.72E-05 | ||
| 1310 | 1.1 | 36.36*** | 22.03 | 40.51 | 1.46E-03 | ||
| 1630 | 2.74 | 42.2*** | 14.38 | 40.68 | 1.55E-04 | ||
| Ethanol | Pdc1p | all isoforms | 7.69 | 20.88* | 24.64 | 46.79 | 1.08E-03 |
| 4854 | 0.65 | 55.53*** | 11.52 | 32.3 | 9.72E-04 | ||
| 1589 | 2.28 | 66.54*** | 11.99 | 19.19 | 1.16E-03 | ||
| 1605 | 6.71 | 39.02*** | 9.49 | 44.78 | 1.15E-04 | ||
| 1606 | 3.64 | 29.71* | 10.56 | 56.1 | 1.65E-05 | ||
| Adh1p | all isoforms | 0.94 | 33.43*** | 23.38 | 42.24 | 7.22E-04 | |
| 4799 | 1.78 | 49.82*** | 17.3 | 31.1 | 1.90E-04 | ||
| 4808 | 0.92 | 27.33** | 28.75 | 43 | 2.45E-04 | ||
| Glycerol | Hor2p | 3129 | 6.76** | 63.72*** | 14.62* | 14.9 | 3.75E-05 |
| Rhr2p | 3003 | 4.08** | 68.49*** | 15.92** | 11.52 | 1.95E-04 | |
| Acetate | Ald6p | 1565 | 23.67 | 18.32 | 19.39 | 38.61 | 8.96E-06 |
a df: degree of freedom. Significance is indicated as follow: * significant at 5%; ** significant at 1%; *** significant at 0.1% (Benjamini-Hochberg correction for multiple testing).