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Extracted ion chromatograms for the 44 observed peptide targets in digested DBS samples with the natural peptides shown in blue and the SIS peptides shown in red. The relative intensity of a natural peptide (LVNEVTEFAK) from albumin, seen at a retention time of 14 min, has been reduced by a factor of 10. The insert shows the 3 transition pairs monitored for a tryptic peptide from alpha-2-HS-glycoprotein.
