Fig. 2.

ELISA-based quantification of recombinant pMAPKs adsorbed to 96-well plates. A–C, Twofold dilution series (relative level 1.0 = ∼400 ng) of pERK (A), pJNK (B), and pp38 (C) were adsorbed onto polystyrene microtiter plates and detected by ELISA using phospho-specific antibodies. The detection saturates at ∼75 ng for pERK (A), ∼400 ng for pJNK (B), and ∼75 ng for pp38 (C). Arrows indicate the quantity of pMAPKs used in the PPase assay. Data are shown as the mean ± S.E. of three independent assay replicates.