Table 2.
Summary of morphological parameters for LHA GAD65-GFP neurons
| RS (n = 9) | FS (n = 6) | LS (n = 9) | LTS (n = 9) | ||
|---|---|---|---|---|---|
| Soma surface (μm2) | 859 ± 118 | 774 ± 113 | 1409 ± 329 | 1234 ± 408 | |
| N_stems | 3.6 ± 0.4 | 4.7 ± 0.8 | 4.1 ± 0.3 | 5.1 ± 0.4 | |
| N_bifs | 17.3 ± 7.7 | 58.7 ± 25.7 | 19.0 ± 9.0 | 20.6 ± 6.4 | |
| N_branch | 40.8 ± 15.4 | 125.7 ± 52.1 | 59.3 ± 25.8 | 57.3 ± 15.6 | |
| N_tips | 20.9 ± 7.7 | 63.3 ± 26.1 | 23.1 ± 9.1 | 25.7 ± 6.4 | |
| Width (μm) | 316 ± 63 | 606 ± 107 | 369 ± 68 | 396 ± 33 | FS>LS, RS |
| Height (μm) | 314 ± 33 | 368 ± 55 | 330 ± 58 | 397 ± 48 | |
| Depth (μm) | 83 ± 7 | 83 ± 14 | 84 ± 10 | 76 ± 4 | |
| Length (μm) | 1910 ± 390 | 4940 ± 1740 | 1920 ± 440 | 2430 ± 370 | FS>LS, RS |
| Surface (μm2) | 4540 ± 880 | 12000 ± 4920 | 4720 ± 950 | 6430 ± 1060 | FS>LS, RS |
| Volume (μm3) | 1440 ± 350 | 3200 ± 1510 | 1620 ± 350 | 2260 ± 450 |
Values are means ± SEM. Statistical testing shown as > = P < 0.05. Soma surface (μm2): surface area of the soma. N_stems: number of processes branching out from soma. N_bifs: number of bifurcations, i.e. branching points. N_branch: number of branches, i.e. segments that lie between two branching points or between one branching point and a termination point. N_tips: number of termination points. Width (μm): maximum distance between two points in the horizontal dimension. Height (μm): maximum distance between two points in the vertical dimension. Depth (μm): maximum depth between two points. Length (μm): sum of the lengths of all components of the structure. Surface (μm2): total surface area of the neuron. Volume (μm3): total volume of the neuron.