Figure 6. Cultured suprachiasmatic nucleus (SCN) and pituitary (PIT) from +/+ mice maintain a phase consistently correlated with the animals circadian time (CT) in vivo, while Afh/Afh SCN and PIT are reset by the culture procedure.

Rayleigh plots show peak PER2::LUC phase for +/+ and Afh/Afh SCN and PIT recorded in PMTs, plotted as either CT (based on behavioural rhythms in DD) or time of peak bioluminescence after culture preparation (time from culture). Circles (open: +/+; filled: Afh/Afh) indicate the phase of peak bioluminescence in individual cultures. Both SCN and PIT from +/+ mice express peak PER2::LUC bioluminescence at a consistent circadian phase, regardless of cull/culture time (peak phase is correlated with CT not with time from culture). However, Afh/Afh SCN and PIT always peak the same number of hours after culture preparation, showing these tissues to be reset by this process (peak phase correlated with time from culture not CT).