Table 1.
Summary data of wheel-running analysis of +/+, Afh/+ and Afh/Afh mice
| +/+ | Afh/+ | Afh/Afh | Significance | |
|---|---|---|---|---|
| LD | ||||
| N | 14 | 8 | 12 (8 rhythmic) | |
| Period (h) | 23.97 ± 0.02 | 23.99 ± 0.02 | 25.30 ± 0.89 | n.s. |
| Strength of rhythm (%V) | 48.73 ± 3.47 | 59.32 ± 7.38 | 32.82 ± 6.00* | P= 0.025 |
| Locomotor activity (Wheel revs h−1) | 674.66 ± 65.92 | 829.95 ± 183.60 | 702.34 ± 115.14 | n.s. |
| Percentage of activity in dark phase | 91.82 ± 2.02 | 89.94 ± 3.65 | 76.82 ± 5.64* | P= 0.042 |
| ZT of nocturnal activity peak | 13.99 ± 0.56 | 13.89 ± 0.52 | 17.50 ± 1.51* | P= 0.019 |
| DD1 | ||||
| N | 14 | 8 | 12 | |
| Period (h) | 23.74 ± 0.06 | 24.11 ± 0.08 | 26.62 ± 0.19* | P= 0.0001 |
| Strength of rhythm (%V) | 45.79 ± 3.08 | 44.31 ± 4.40 | 39.41 ± 2.78 | n.s. |
| Locomotor activity (Wheel revs h−1) | 714.99 ± 70.63 | 773.71 ± 197.38 | 784.59 ± 85.48 | n.s. |
| Phase shift on transfer to DD (min) | 43.94 ± 8.77 | 4.80 ± 17.64 | −202.95 ± 46.86* | P= 0.0001 |
| Alpha duration (h) | 12.67 ± 0.74 | 14.94 ± 1.08 | 15.54 ± 0.60 | P= 0.017 |
| Alpha duration as a percentage DD1-10 period | 53.38 ± 3.04 | 61.85 ± 4.52 | 58.44 ± 0.60 | n.s. |
| Percentage of activity in alpha in DD1-10 | 90.55 ± 1.55 | 95.32 ± 0.69 | 89.25 ± 2.19 | n.s. |
| DD2 | ||||
| N | 14 | 6 | 8 | |
| Period DD (h) | 23.79 ± 0.12 | 24.30 ± 0.14 | 26.36 ± 0.16* | P= 0.0001 |
| Strength of rhythm in DD10−20 (%V) | 38.83 ± 4.82 | 40.00 ± 5.94 | 37.33 ± 3.16 | n.s. |
| Rel. change in %V (DD10−20 :DD1-10) | −0.16 ± 0.07 | −0.13 ± 0.07 | −0.07 ± 0.10 | n.s. |
Wheel-running parameters of rhythmic mice measured in LD, DD1-10 and DD10−20. %V= percentage of variance explained by the dominant component of the rhythm; rev h−1= revolutions per hour; alpha = consolidated active phase. Values are expressed as mean ± SEM. Bold underlined values are statistically different from +/+ mice. *Indicates statistically significant differences between Afh/+ and Afh/Afh groups (one-way ANOVA followed by Tukey post hoc analyses; P < 0.05; significance column shows results of ANOVA). DD, constant darkness; LD, light/dark; ZT, Zeitgeber time.