Table 2.
Summary of drinking parameters in +/+, Afh/+ and Afh/Afh mice
| +/+ | Afh/+ | Afh/Afh | Significance | |
|---|---|---|---|---|
| LD | ||||
| N | 5 | 4 | 5 | |
| Period (h) | 24.00 ± 0.07 | 24.19 ± 0.02 | 24.75 ± 0.60 | n.s. |
| Strength of rhythm (%V) | 45.40 ± 6.52 | 27.66 ± 3.98 | 23.10 ± 2.44 | P= 0.014 |
| Total water intake (ml day−1) | 5.54 ± 0.49 | 5.54 ± 0.44 | 4.77 ± 0.39 | n.s. |
| Percentage of drinking activity in dark phase | 74.44 ± 4.97 | 67.81 ± 4.50 | 62.80 ± 4.74 | n.s |
| DD | ||||
| N | 5 | 5 | 6 | |
| Period (h) | 23.85 ± 0.04 | 24.25 ± 0.13 | 25.67 ± 0.21* | P= 0.0001 |
| Strength of rhythm (%V) | 23.74 ± 4.44 | 18.80 ± 2.11 | 20.88 ± 2.25 | n.s. |
| Total water intake (ml day−1) | 5.92 ± 0.63 | 5.30 ± 0.69 | 5.35 ± 0.47 | n.s. |
| Alpha duration (h) | 9.45 ± 0.59 | 9.30 ± 0.70 | 11.78 ± 0.61* | P= 0.026 |
| Alpha duration as a percentage DD period | 39.62 ± 2.54 | 38.40 ± 3.08 | 45.80 ± 2.04 | n.s. |
| Percentage of drinking activity in alpha | 60.22 ± 2.54 | 54.52 ± 4.45 | 63.01 ± 1.98 | n.s. |
Parameters of drinking and water consumption of mice classified as rhythmic in LD and DD. %V= percentage of variance explained by the dominant component of the rhythm; alpha = main active phase. Values are expressed as mean ± SEM. Bold underlined values are statistically different from +/+ mice. *Indicates statistically significant differences between Afh/+ and Afh/Afh groups (one-way ANOVA followed by Tukey post hoc analyses; P < 0.05). -values refer to the first level of ANOVA. DD, constant darkness; LD, light/dark.