Table 4.
Comparison of rhythms in PER2::LUC in +/+, Afh/+ and Afh/Afh mice from slice cultures recorded in PMTs
| N | % slices rhythmic | Period (h) | Amplitude (cpm) | |
|---|---|---|---|---|
| SCN | ||||
| +/+ | 24 | 100 | 24.04 ± 0.25 | 15076 ± 2512 |
| Afh/+ | 10 | 100 | 24.61 ± 0.37 | 7847 ± 3275 |
| Afh/Afh | 21 | 95.2 | 25.61 ± 0.27 | 7123 ± 1484 |
| PIT | ||||
| +/+ | 24 | 100 | 23.59 ± 0.17 | 20924 ± 3000 |
| Afh/+ | 13 | 84.6 | 24.68 ± 0.9 | 5995 ± 1992 |
| Afh/Afh | 15 | 93.3 | 26.63 ± 0.44* | 2311 ± 417* |
| Arc complex | ||||
| +/+ | 25 | 88 | 23.3 ± 0.35 | 830 ± 230 |
| Afh/+ | 14 | 71.4 | 24.3 ± 0.56 | 796 ± 246 |
| Afh/Afh | 21 | 52.4 | 24.9 ± 0.49 | 168 ± 37 |
| DMH | ||||
| +/+ | 25 | 84 | 23.13 ± 0.56 | 587 ± 127 |
| Afh/+ | 14 | 78.6 | 22.88 ± 0.74 | 629 ± 165 |
| Afh/Afh | 21 | 52.4 | 26.16 ± 0.91* | 229.9 ± 40 |
| Hb | ||||
| +/+ | 12 | 66.7 | 22.11 ± 0.10 | 514 ± 143 |
| Afh/+ | 5 | 80 | 23.91 ± 0.98 | 417 ± 117 |
| Afh/Afh | 14 | 50 | 24.68 ± 1.11 | 205 ± 103 |
Values are expressed as mean ± SEM. Bold underlined values are statistically different from +/+ mice. *Indicates statistically significant differences between Afh/+ and Afh/Afh groups. Arc, arcuate nucleus; DMH, dorsomedial hypothalamus; Hb, habenula; PIT, pituitary; SCN, suprachiasmatic nucleus.