Table 5.
Summary of circadian rhythms of PER2::LUC expression in hypothalamic tissues recorded using a bioluminescence imaging camera
| N | % slices rhythmic | Period (h) | % increase in period vs. +/+ | Amplitude | Rate of damping (days) | |
|---|---|---|---|---|---|---|
| Arc | ||||||
| +/+ | 10 | 100 | 21.4 ± 0.7 | 12.5 | 1413 ± 192 | 5.8 ± 0.56 |
| Afh/Afh | 11 | 82 | 24.0 ± 0.5** | 410 ± 186* | 2.5 ± 0.56*** | |
| DMH | ||||||
| +/+ | 10 | 100 | 21.2 ± 0.4 | 13.4 | 772 ± 185 | 5.7 ± 0.47 |
| Afh/Afh | 11 | 63.6 | 24.0 ± 1.0* | 416 ± 167 | 2.5 ± 0.70** | |
| ME | ||||||
| +/+ | 10 | 100 | 23.8 ± 0.4 | 9.1 | 2470 ± 1420 | 7.0 ± 0.43 |
| Afh/Afh | 10 | 70 | 26.0 ± 0.8* | 1061 ± 654 | 4.2 ± 0.8** | |
| Epe | ||||||
| +/+ | 10 | 100 | 23.5 ± 0.6 | 10.3 | 2090 ± 1364 | 6.6 ± 0.76 |
| Afh/Afh | 10 | 80 | 26.0 ± 0.8* | 354 ± 117 | 3.3 ± 0.67** | |
| SCN | ||||||
| +/+ | 5 | 100 | 23.7 ± 0.3 | 9.0 | 5447 ± 155 | n/a |
| Afh/Afh | 5 | 100 | 25.8 ± 1.4* | 3307 ± 964 | n/a | |
Values are expressed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 unpaired t test. Arc, arcuate nucleus; DMH, dorsomedial hypothalamus; Epe, ependymal cell layer of the 3rd ventricle; ME, median eminence; SCN, suprachiasmatic nucleus.