Table 1.
AP parameters following the 1st and 20th AP of a train
| ANOVA main effects P | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cell type | Control | SNL4 | SNL5 | Injury | AP repeat | Injury × AP repeat | ||
| N | Ai | 55 | 25 | 41 | ||||
| Ao | 91 | 44 | 28 | |||||
| C | 31 | 6 | 12 | |||||
| Diameter (μm) | Ao | 38.9 ± 0.8 | 39.3 ± 1.0 | 37.7 ± 1.6 | 0.73 | |||
| Ai | 41.7 ± 0.8 | 39.4 ± 1.8 | 37.6 ± 0.9* | 0.007 | ||||
| C | 32.5 ± 1.7 | 33.7 ± 2.0 | 31.2 ± 1.7 | 0.61 | ||||
| Initial RMP | Ao | −60.8 ± 0.9 | −62.7 ± 1.0 | −61.3 ± 1.0 | 0.4 | |||
| Ai | −65.7 ± 0.9 | −63.2 ± 1.3 | −58.6 ± 0.8 | <0.001 | ||||
| C | −66.7 ± 1.5 | −65.8 ± 3.0 | −66.1 ± 1.4 | 0.93 | ||||
| CV | Ao | 1st (m s−1) | 22.1 ± 0.9 | 20.5 ± 1.2 | 16.5 ± 1.1 | 0.006 | <0.001 | 0.07 |
| 20th (m s−1) | 19.5 ± 0.8§§ | 18.7 ± 1.2§§ | 14.8 ± 1.0§§ | |||||
| Δ (%) | −11.4 ± 0.9 | −9.3 ± 1.5 | −10.0 ± 1.8 | 0.4 | ||||
| Ai | 1st (m s−1) | 17.9 ± 1.1 | 14.0 ± 1.3 | 15.2 ± 1.0 | 0.1 | <0.001 | 0.26 | |
| 20th (m s−1) | 15.7 ± 0.9§§ | 12.4 ± 1.0§§ | 14.08 ± 1.0§§ | |||||
| Δ (%) | −9.9 ± 1.6 | −10.5 ± 2.0 | −7.5 ± 1.2 | 0.37 | ||||
| C | 1st (m s−1) | 0.8 ± 0.0 | 0.8 ± 0.1 | 0.9 ± 0.1 | 0.46 | 0.001 | 0.24 | |
| 20th (m s−1) | 0.7 ± 0.1 | 0.7 ± 0.0 | 0.8 ± 0.0§ | |||||
| Δ (%) | −8.5 ± 3.8 | −11.5 ± 2.7 | −15.9 ± 6.2 | 0.56 | ||||
| APamp | Ao | 1st (mV) | 69.2 ± 1.5 | 75.7 ± 1.8* | 71.4 ± 2.3 | 0.07 | 0.55 | 0.24 |
| 20th (mV) | 70.9 ± 1.5 | 75.5 ± 2.1* | 71.0 ± 2.4 | |||||
| Δ (%) | 2.8 ± 1.21 | −0.0 ± 1.90 | −0.3 ± 1.67 | 0.26 | ||||
| Ai | 1st (mV) | 80.9 ± 1.7 | 85.7 ± 2.1 | 71.7 ± 1.8† | <0.001 | <0.001 | 0.001 | |
| 20th (mV) | 79.6 ± 1.7 | 80.8 ± 3.1 | 62.2 ± 2.6§§**†† | |||||
| Δ (%) | −1.2 ± 1.5 | −5.8 ± 2.5* | −13.6 ± 2.3** | <0.001 | ||||
| C | 1st (m s−1) | 87.2 ± 2.2 | 85.0 ± 4.4 | 79.7 ± 3.2 | 0.34 | 0.95 | 0.21 | |
| 20th (m s−1) | 82.2 ± 2.4 | 78.9 ± 6.0 | 77.8 ± 4.0 | |||||
| Δ (%) | −4.2 ± 2.5 | −0.8 ± 3.4 | −2.9 ± 4.3 | 0.84 | ||||
| APd | Ao | 1st (ms) | 0.8 ± 0.0 | 0.72 ± 0.0 | 1.0 ± 0.1 | 0.005 | <0.001 | 0.15 |
| 20th (ms) | 1.1 ± 0.1§§ | 1.0 ± 0.1§§ | 1.3 ± 0.1§§ | |||||
| Δ (%) | 48.7 ± 2.9 | 41.6 ± 4.1 | 30.8 ± 4.4** | 0.007 | ||||
| Ai | 1st (ms) | 1.0 ± 0.1 | 1.0 ± 0.1 | 1.6 ± 0.1* | <0.001 | <0.001 | 0.8 | |
| 20th (ms) | 1.5 ± 0.1§§ | 1.5 ± 0.1§§ | 2.0 ± 0.1§§* | |||||
| Δ (%) | 47.3 ± 5.4 | 39.9 ± 7.1 | 31.1 ± 3.3 | 0.06 | ||||
| C | 1st (m s−1) | 3.3 ± 0.2 | 3.1 ± 0.2 | 3.6 ± 0.3 | 0.83 | 0.03 | 0.25 | |
| 20th (m s−1) | 2.8 ± 0.1§§ | 3.2 ± 0.3 | 3.2 ± 0.4 | |||||
| Δ (%) | −13.9 ± 4.0 | −6.8 ± 3.9 | −7.6 ± 6.6 | 0.59 | ||||
Values are expressed as mean ± SEM. Injury effect P indicates the ANOVA main effect of injury for the parameter in that row. For post hoc comparisons: different from Control *P < 0.05, **P < 0.01; different from SNL4 †P < 0.05, ††P < 0.01; 20th different from 1st §P < 0.05, §§P < 0.01. AP, action potential; APamp, action potential amplitude; APd, action potential duration; CV, conduction velocity; n, number of cells; RMP, resting membrane potential; SNL, spinal nerve ligation; SNL4, 4th lumbar ganglion after SNL; SNL5, 5th lumbar ganglion after SNL.