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. 2013 Jan 10;121(10):1690–1700. doi: 10.1182/blood-2012-07-445197

Figure 3.

Figure 3

Emergence of neutrophil-DC hybrids in various organs under inflammatory conditions. Single cell suspensions were prepared from ear skin samples harvested from KC-Tie2 TG mice or age-matched WT mice (3 mice per panel) (A), from lung samples harvested from BALB/c mice (3 mice per panel) 48 hours after intratracheal instillation of GC frass or PBS alone (B), or from skin-draining LN samples harvested from C57BL/6 mice (5 mice per panel) 24 hours after subcutaneous inoculation of live E. coli or PBS alone (C). Data shown are the numbers (means ± SD) of Ly6G+/CD11c−/MHC II− neutrophils, Ly6G+/CD11chigh/MHC II+ neutrophil-DC hybrids, and Ly6G−/CD11chigh/MHC II+ traditional DCs. *P < .05; **P < .01 between the indicated samples. (D) The same LN samples harvested after E. coli injection were examined for expression of the indicated leukocyte markers within the Ly6G+/CD11chigh gated population. Data are representative of 5 independent experiments.