Figure 1.

The Proximal Promoter of Human F9

The major transcription start site (TSS) is indicated by an arrow and designated +1. Point mutations associated with hemophilia B Leyden are indicated above the sequence. The transcription factors known to bind this region are shown, and the consensus sequences that they recognize are aligned below the sequence. DNA-binding consensus sequences were determined from the ChIP-Seq data reported here (ArrayExpress accession number E-MTAB-890) and previously (ArrayExpress E-TABM-722)⁹ with the default settings of MEME and the following parameters: -revcomp -maxw 20 -minw 6 -nmotifs 5. We selected the top 500 peaks ordered by input-corrected read depth and used the 25 base pairs centered on the identified summit of the peak as our input for the motif-discovery analysis. The position weight matrices are consistent with previously determined DNA-binding consensuses for these transcription factors.^9–11 The following protein abbreviations are used: HNF4α, hepatocyte nuclear factor 4 alpha; C/EBPα, CCAAT/enhancer-binding protein alpha; and OC1/2, ONECUT1/2.