Figure 2.

Effect of ADAMTS7 Genotype on VSMC Migration

(A) Primary cultures of VSMCs were subjected to double fluorescent immunostaining for the smooth muscle α-actin (SMA, red) and ADAMTS7 (green) and nucleus staining with propidium iodide (blue). Representative images are shown.

(B) Primary cultures of VSMCs of the A/A, A/G, or G/G genotype for rs3825807 were subjected to scratch assay by a commonly used method.¹¹ Column chart shows migration distances (mean ± SEM) of VSMCs of the A/A, A/G, or G/G genotype for rs3825807 (n = 5 different donor cell preparations for each genotype). ^∗p < 0.05 comparing A/A and G/G genotype group.

(C and D) Primary cultures of VSMCs of the A/A or G/G genotype were subjected to scratch (C) and migration (D) assays, without or with medium swapping at the outset of the assay (i.e., at hour 0 of the assay, the culture medium of G/G genotype cells was replaced by A/A genotype cell conditioned medium, and vice versa). Column chart shows migration distances or migrated cell numbers (mean ± SEM) (n = 3 different donor cell preparations for each genotype). ^∗p < 0.05 comparing A/A genotype cells without medium swapping and G/G genotype cells without medium swapping; #p < 0.05 comparing G/G genotype cells with A/A genotype cell conditioned medium versus G/G genotype cells without medium swapping.