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. 2013 Mar 7;92(3):366–374. doi: 10.1016/j.ajhg.2013.01.012

Figure 4.

Figure 4

Effect of ADAMTS7 Genotype on the Amount of Cleaved ADAMTS7 Prodomain in VSMC Conditioned Media

(A) A representative image of immunoblot (IB) analysis of cleaved ADAMTS7 prodomain in media conditioned by VSMCs of the A/A, A/G, or G/G genotype for rs3825807. The same amount (20 μg) of proteins for each genotype was loaded. Data shown in column chart are mean (±SEM) values of cleaved ADAMTS7 prodomain band intensity in immunoblots (n = 5 different donor cell preparations for each genotype). ∗p < 0.05 comparing A/A and G/G genotypes.

(B) A representative image of immunoblot analysis of cleaved ADAMTS7 prodomain in lysates of VSMCs of the A/A or G/G genotype for rs3825807. The same amount (20 μg) of proteins for each genotype was loaded. Data shown in column chart are mean (±SEM) values of full-length ADAMTS7 band intensity in immunoblots (n = 3 different donor cell preparations for each genotype).