Table 1.
Survival of A and H cultures of M. tuberculosis in the acidic Wayne model after 7, 14, and 21 days of exposure to various drug combinations, as estimated by regrowth in liquid medium by using the BACTEC MGIT 960 systema
| Combination | DTP ± SD by days of exposure at growth stage shownb: |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A5 |
H5 |
H12 |
H19 |
|||||||||
| 7 | 14 | 21 | 7 | 14 | 21 | 7 | 14 | 21 | 7 | 14 | 21 | |
| Control | 2 ± 1 | 2 ± 1 | 2 ± 1 | 3 ± 1 | 3 ± 1 | 4 ± 1 | 3 ± 1 | 4 ± 2 | 5 ± 1 | 4 ± 1 | 5 ± 1 | 5 ± 1 |
| R-I-Z-E | 20 ± 4 | 27 ± 3 | >100 | 19 ± 1 | 23 ± 5 | 24 ± 5 | 15 ± 1 | 26 ± 4 | >100 | 18 ± 3 | 32 ± 10 | >100 |
| R-MX-AK | 15 ± 2 | 24 ± 4 | >100 | 16 ± 2 | 23 ± 1 | >100 | 19 ± 2 | 51 ± 4 | >100 | 19 ± 2 | 22 ± 1 | >100 |
| R-MX-AK-MZ | 21 ± 7 | 18 ± 3 | >100 | 17 ± 2 | 21 ± 7 | >100 | 19 ± 5 | 28 ± 6 | >100 | 17 ± 2 | >100 | >100 |
| R-MX-AK-NZ | 17 ± 3 | 24 ± 3 | >100 | 15 ± 1 | 22 ± 2 | >100 | 16 ± 2 | >100 | >100 | 18 ± 1 | >100 | >100 |
| R-MX-AK-NC | 16 ± 2 | 19 ± 2 | 20 ± 1 | 13 ± 2 | 18 ± 1 | >100 | 15 ± 2 | 27 ± 3 | >100 | 19 ± 1 | 27 ± 1 | >100 |
| R-MX-AK-PA | 38 ± 8 | >100 | >100 | 26 ± 4 | >100 | >100 | 20 ± 2 | >100 | >100 | 24 ± 4 | >100 | >100 |
a
Five-day-old aerobic (A5) cultures, and 5-, 12-, and 19-day-old hypoxic (H5, H12, and H19, respectively) cultures were incubated with various combinations of drugs for 7, 14, and 21 days. R, rifampin; I, isoniazid; Z, pyrazinamide; E, ethambutol; AK, amikacin; MX, moxifloxacin; MZ, metronidazole; NZ, nitazoxanide; NC, niclosamide; PA, PA-824. The drug concentrations used were 8, 2, 100, 4, 8, 4, 8, 10, 0.3, and 2 μg/ml, respectively.
b
Means ± standard deviations from three experiments are shown.